Pneumatically Controlled Non-Equibiaxial Cell Stretching Device with Live-cell Imaging

Abstract

AbstractObjectiveAdherent cell behavior is influenced by a complex interplay of factors, including chemical and mechanical signals.In vitroexperiments that mimic the mechanical environment experienced by cellsin vivoare crucial for understanding cellular behavior and the progression of disease. In this study, we developed and validated a low-cost pneumatically-controlled cell stretcher with independent control of strain in two directions of a membrane, enabling unequal biaxial stretching and realtime microscopy during actuation.MethodsThe stretching was achieved by two independent pneumatic channels controlled by electrical signals. We used finite element simulations to compute the membrane’s strain field and particle tracking algorithms based on image processing techniques to validate the strain fields and measure the cell orientation and morphology.ResultsThe device can supply uniaxial, equibiaxial, and unequal biaxial stretching up to 15% strain in each direction at a frequency of 1Hz, with a strain measurement error of less than 1%. Through live cell imaging, we determined that distinct stretching patterns elicited differing responses and alterations in cell orientation and morphology, particularly in terms of cell length and area.ConclusionThe device successfully provides a large, uniform, and variable strain field for cell experiments, while also enabling real-time, live cell imaging.SignificanceThis scalable, low-cost platform provides mechanical stimulation to cell cultures by independently controlling strains in two directions. This could contribute to a deeper understanding of cellular response to biorealistic strains and could be useful for futurein vitrodrug testing platforms.