D27-LIKE1 carotenoid isomerase has a preference towards trans/cis and cis/cis conversions in Arabidopsis

Abstract

AbstractCarotenoids are colourful isoprenoids that contribute to a variety of physiological processes in plants. They also function as biosynthesis precursors of abscisic acid (ABA) and strigolactones (SLs). SL biosynthesis starts with the enzymatic conversion of all-trans-β-carotene to 9-cis-β-carotene by the DWARF27 (D27) isomerase. In Arabidopsis, D27 has two closely related paralogs, D27-LIKE1 and D27-LIKE2 which were predicted to be β-carotene-isomerases. Here we characterised D27-LIKE1 and identified some key aspects of its function. Arabidopsis d27-like1-1 mutant does not display any SL or karrikin-deficient traits, however, it exhibits a substantially higher 9-cis-violaxanthin content. In vitro feeding assays with recombinant D27-LIKE1 revealed that the protein exhibits affinity to all β-carotene isoforms but with an exclusive preference towards trans/cis conversions and the interconversion between 9-cis, 13-cis and 15-cis-β-carotene forms. Feeding experiments with zeaxanthin and violaxanthin isomers revealed that D27-LIKE1 accepts these xanthophylls as substrates. The remarkably higher 9-cis-violaxanthin content of the mutant is accompanied by a slightly higher ABA level. Finally, we presented evidence that D27-LIKE1 mRNA is phloem mobile and D27-LIKE1 is an ancient isomerase with long evolutionary history. In summary, we demonstrated that D27-LIKE1 is a carotenoid isomerase with multi-substrate specificity and has a characteristic preference towards the catalysation of cis/cis interconversion of carotenoids. Therefore, D27-LIKE1 is a potential regulator of carotenoid cis pools and eventually, SL and ABA biosynthesis pathways.