The males of the parasitoid wasp,Nasonia vitripennis, can identify which fly hosts contain females

Abstract

The reproductive success of a male is limited by the number of females it can mate with. Thus, males deploy elaborate mate-finding strategies to maximize access to females. In the haplodiploid wasp genus, Nasonia, which are parasitoids of cyclorrhaphous flies, mate-finding is restricted to the natal patch, where males compete for access to females. This study investigates whether there are any additional mate finding strategies of males, especially, whether they can identify the presence of adult females which are still inside the fly host. Results reveal that only one out of the four species, N. vitripennis, can distinguish which hosts specifically have adult female wasps indicating a species-specific unique mate-finding capability. Behavioral assays revealed that the cues used by N. vitripennis males are olfactory in nature and not auditory or visual. GC-MS analyses show that these olfactory cues are female-specific cuticular hydrocarbons (CHCs), possibly emanating from within the fly puparium. Further assays indicated that N. vitripennis males can also detect differences in the concentrations of compounds to identify female-specific cues from male-specific ones. This study, therefore, uncovers a previously unknown mate-finding strategy in one of the most widely studied parasitoid wasp.