Growing Hybridomas

Author:

Greenfield Edward A.

Abstract

This introduction discusses the techniques used to grow and maintain myeloma and hybridoma cell lines, the production and collection of monoclonal antibodies, and methods for drug selection used in hybridoma work.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

Reference38 articles.

1. Use of Hoechst 33258 fluorochrome for detection of mycoplasma contamination in cell cultures: Development of a technique based on simultaneous fixation and staining;Boll Ist Sieroter Milan,1980

2. Rapid isolation of monoclonal hybridoma cultures by a ‘fusion-cloning’ method: the requirement for aminopterin

3. Production of monoclonal antibodies in serum free medium

4. Ammonium Sulfate Fractionation of Antibodies

5. Growth of NS0 Cells in Protein-Free, Chemically Defined Medium

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Generating Monoclonal Antibodies;Cold Spring Harbor Protocols;2022-08

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