Abstract
AbstractA highly sensitive immunoassay with sub-picogram limit of detection for fentanyl and a wide range of fentanyl analogs has been developed, using a novel high affinity antibody fused with NanoLuc, a small-size luciferase that can emit strong and stable luminescence. When applied to unaltered clinical human urine samples, the assay has sub-picogram limit of detection for fentanyl, with results fully concordant with gold standard liquid chromatography-mass spectrometry. When applied to environmental samples, the assay can detect as low as 0.25 pg fentanyl per inch2environment surface. Assay turn-around-time is less than 1 hour, with inexpensive equipment needed and potential for high throughput automation or in-field screening. This novel assay has broad potential applications in clinical, environmental, occupational and forensic scenarios by detecting trace amount of fentanyl and its analogs to keep frontline workers safe.
Publisher
Cold Spring Harbor Laboratory
Reference39 articles.
1. National Vital Statistics System. Provisional Drug Overdose Death Counts. 2023; Available from: https://www.cdc.gov/nchs/nvss/vsrr/drug-overdose-data.htm. Accessed October 30, 2023.
2. The Emerging Role of Toxic Adulterants in Street Drugs in the US Illicit Opioid Crisis;Public Health Rep,2020
3. Illicit fentanyls in the opioid street market: desired or imposed?;Addiction,2019
4. Studies on 1-(2-phenethyl)-4-(N-propionylanilino) piperidine (fentanyl) and its related compounds. VI. Structure-analgesic activity relationship for fentanyl, methyl-substituted fentanyls and other analogues;Forensic Toxicology,2008
5. Fentanyl, fentanyl analogs and novel synthetic opioids: A comprehensive review;Neuropharmacology,2018