A Translaminar Spacetime Code Supports Touch-Evoked Traveling Waves

Abstract

SUMMARYLinking sensory-evoked traveling waves to underlying circuit patterns is critical to understanding the neural basis of sensory perception. To form this link, we performed simultaneous electrophysiology and two-photon calcium imaging through transparent NeuroGrids and mapped touch-evoked cortical traveling waves and their underlying microcircuit dynamics. In awake mice, both passive and active whisker touch elicited traveling waves within and across barrels, with a fast early component followed by a variable late wave that lasted hundreds of milliseconds post-stimulus. Strikingly, late-wave dynamics were modulated by stimulus value and correlated with task performance. Mechanistically, the late wave component was i) modulated by motor feedback, ii) complemented by a sparse ensemble pattern across layer 2/3, which a balanced-state network model reconciled via inhibitory stabilization, and iii) aligned to regenerative Layer-5 apical dendritic Ca2+events. Our results reveal a translaminar spacetime pattern organized by cortical feedback in the sensory cortex that supports touch-evoked traveling waves.GRAPHICAL ABSTRACT AND HIGHLIGHTSWhisker touch evokes both early- and late-traveling waves in the barrel cortex over 100’s of millisecondsReward reinforcement modulates wave dynamicsLate wave emergence coincides with network sparsity in L23 and time-locked L5 dendritic Ca2+spikesExperimental and computational results link motor feedback to distinct translaminar spacetime patterns