Temperature-responsive control ofPseudomonas aeruginosavirulence determinants through the stabilization of quorum sensing transcriptional regulator RhlR

Abstract

AbstractThe versatile bacteriumPseudomonas aeruginosathrives in diverse environments and is notably recognized for its role as an opportunistic pathogen. In line with its adaptability,P. aeruginosaproduces various exoproducts crucial for survival and virulence, several of which regulated through quorum sensing (QS). These factors are also regulated in response to environmental cues, such as temperature changes. As a pathogen,P. aeruginosais generally thought to activate its virulence factors at temperatures akin to warm-blooded hosts rather than environmental temperatures. Recent studies elucidated the functional structure of the QS transcriptional regulator RhlR, which depends on the stabilizing effects of its cognate autoinducing ligand,N-butanoyl-L-homoserine lactone (C4-HSL), and of the moonlighting chaperone PqsE. Given the influence of temperature on biomolecular dynamics, we investigated how it affects RhlR activity using the RhlR-regulatedphzA1promoter as a proxy. Unexpectedly, we found that RhlR activity is higher at 25°C than at 37°C. This temperature-dependent regulation likely stems from altered RhlR turnover, with the presence of PqsE extending RhlR activity tenfold from its basal level at 37°C to that observed at 25°C. This lower, environmental-like temperature promotes increased affinity between RhlR and C4-HSL, a trait significantly compromised in the absence of PqsE. These results suggest that this response depends on the structural integrity of the complex, indicating that temperature functions as an additional regulating and stabilizing factor of RhlR function. Accordingly, lower growth temperature fails to increase the activity of a structurally stabilized version of RhlR. The thermoregulation aspect of RhlR activity and signalling impacts the virulence profile of a mutant unable to produce C4-HSL, underscoring its significance in bacterial behaviours and potentially conferring an evolutionary advantage.Author SummaryPseudomonas aeruginosais recognized for its capacity to colonize vastly different environments, thereby encountering a range of temperatures. The bacterium’s ability to adapt to these settings necessitates finely regulated gene expression. Within this regulatory framework lies quorum sensing (QS), the intercellular communication system used byP. aeruginosato orchestrate the expression of genes responsible for producing diverse exoproducts, including the blue phenazine pyocyanin. RhlR primarily governs the expression of genes required for pyocyanin production, including thephz1operon. Unlike other QS regulators, RhlR possesses a distinctive characteristic – in addition to its cognate signalling ligand C4-HSL, it depends on the presence of the chaperone-like protein PqsE for stability and activity. This intrinsic instability implies that RhlR may be susceptible to external influences that can modulate its function. Indeed, a lower culture temperature, akin to an environmental-like condition, induces the transcription of thephz1operon, used as a proxy for RhlR activity. Using a combination of genetic approaches, we present evidence that this thermoregulation is due to an impact on the stability of the RhlR/C4-HSL/PqsE complex. We further show the biological effect of this regulation mechanism in an infection setting, which could underscore a relevant role for other bacterial behaviours.