Widespread changes in gene expression accompany body size evolution in nematodes

Abstract

Body size is a fundamental trait that drives multiple evolutionary and ecological patterns.Caenorhabditis inopinatais a fig-associated nematode that is exceptionally large relative to other members of the genus, includingC. elegans. We previously showed thatC. inopinatais large primarily due to postembryonic cell size expansion that occurs during the larval-to-adult transition. Here, we describe gene expression patterns inC. elegansandC. inopinatathroughout this developmental period to understand the transcriptional basis of body size change. We performed RNA-seq in both species across the L3, L4, and adult stages. Most genes are differentially expressed across all developmental stages, consistent withC. inopinata’s divergent ecology and morphology. We also used a model comparison approach to identify orthologs with divergent dynamics across this developmental period between the two species. This included genes connected to neurons, behavior, stress response, developmental timing, and small RNA/chromatin regulation. Multiple hypodermal collagens were also observed to harbor divergent developmental dynamics across this period, and genes important for molting and body morphology were also detected. Genes associated with TGF-β signaling revealed idiosyncratic and unexpected transcriptional patterns given their role in body size regulation inC. elegans. Widespread transcriptional divergence between these species is unexpected and may be a signature of the ecological and morphological divergence ofC. inopinata. Alternatively, transcriptional turnover may be the rule in theCaenorhabditisgenus, indicative of widespread developmental system drift among species. This work lays the foundation for future functional genetic studies interrogating the bases of body size evolution in this group.