Abstract
The establishment of an extracellular vesicle (EV) functional assay is essential for defining the bioactivity, quality, batch-to-batch reproducibility and stability of EV preparations to facilitate subsequent applications as therapeutics or carriers for cell-free therapies. The high heterogeneity of EV preparations requires a sensitive test that supports gold-standard-methods reported in the Minimal Information for Studies of Extracellular Vesicles (MISEV, 2018) guidelines for the functional characterization of EVs. Here, we developed a ubiquitous enzymatic-based assay for EVs, a single-step functional analysis to predict their potency that simultaneously allows a quantitative measure of EV-bioactivity and vesicle integrity, using small-size EV samples. In particular, we set up and validated this robust and simple test using nanoalgosomes as EV-model system, selecting a specific substrate for enzymes present the vesicular cargo of several type of EVs, including mammalian’s one. We also explored the proposed assay’s potential for EV-quality check after different EV-isolation, storage, and loading methods.
Publisher
Cold Spring Harbor Laboratory
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献