A high-resolution haplotype pangenome uncovers somatic hybridization, recombination and intercontinental migration in oat crown rust

Abstract

AbstractBasidiomycetes like rust fungi have complex reproductive cycles and dikaryotic life stages which influence their population structure and evolution.Puccinia coronataf. sp.avenae(Pca), the causal agent of oat crown rust, is a pathogen of global economic importance. To investigate the genetic diversity of the species, as well as the role of mating type system and nuclear exchange (somatic hybridization) in host adaptation ofPcawe acquired whole genome sequencing data of Taiwanese and Australian isolates, adding to existing data for USA and South African populations. An atlas of 30 chromosome-level, fully-phased nuclear haplotypes from six USA isolates and nine Australian isolates was generated to capture the genomic composition of key oat crown rust lineages. This study provides evidence of nuclear exchange and recombination of haplotypes in both the USA and AustralianPcapopulations as mechanisms for the introduction of genetic diversity. The limitations of assuming clonal evolutionary history from virulence phenotyping is demonstrated by the detection of either sexual or cryptic genetic recombination in thePcaAustralian population. Overall, the characterization of intercontinental migration ofPcaat the haplotype level provides further impetus for molecular monitoring of rust pathogen populations on a global scale.