Mitochondrial reverse electron transport in myeloid cells perpetuates neuroinflammation

Author:

Peruzzotti-Jametti L.,Willis C.M.,Hamel R.,Krzak G.,Reisz J.A.,Prag H.A.,Wu V.,Xiang Y.,van den Bosch A.M.R.,Nicaise A.M.,Roth L.,Bates G.R.,Huang H.,Vincent A.E.,Frezza C.,Viscomi C.,Marioni J.C.,D’Alessandro A.,Takats Z.,Murphy M.P.,Pluchino S.

Abstract

ABSTRACTSustained smouldering, or low grade, activation of myeloid cells is a common hallmark of several chronic neurological diseases, including multiple sclerosis (MS)1. Distinct metabolic and mitochondrial features guide the activation and the diverse functional states of myeloid cells2. However, how these metabolic features act to perpetuate neuroinflammation is currently unknown. Using a multiomics approach, we identified a new molecular signature that perpetuates the activation of myeloid cells through mitochondrial complex II (CII) and I (CI) activity driving reverse electron transport (RET) and the production of reactive oxygen species (ROS). Blocking RET in pro-inflammatory myeloid cells protected the central nervous system (CNS) against neurotoxic damage and improved functional outcomes in animal disease modelsin vivo. Our data show that RET in myeloid cells is a potential new therapeutic target to foster neuroprotection in smouldering inflammatory CNS disorders3.

Publisher

Cold Spring Harbor Laboratory

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