SnFFPE-Seq: towards scalable single nucleus RNA-Seq of formalin-fixed paraffin-embedded (FFPE) tissue-Reference-Cited by-同舟云学术

SnFFPE-Seq: towards scalable single nucleus RNA-Seq of formalin-fixed paraffin-embedded (FFPE) tissue

Published:2022-08-25 Issue: Volume: Page:
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Author:

Chung Hattie^ORCID,Melnikov Alexandre^ORCID,McCabe Cristin^ORCID,Drokhlyansky Eugene^ORCID,Van Wittenberghe Nicholas^ORCID,Magee Emma M.^ORCID,Waldman Julia^ORCID,Spira Avrum^ORCID,Chen Fei,Mazzilli Sarah^ORCID,Rozenblatt-Rosen Orit^ORCID,Regev Aviv^ORCID

Abstract

AbstractProfiling cellular heterogeneity in formalin-fixed paraffin-embedded (FFPE) tissues is key to characterizing clinical specimens for biomarkers, therapeutic targets, and drug responses. Here, we optimize methods for isolating intact nuclei and single nucleus RNA-Seq from FFPE tissues in the mouse brain, and demonstrate a pilot application to a human clinical specimen of lung adenocarcinoma. Our method opens the way to broad applications of snRNA-Seq to archival tissues, including clinical samples.

Publisher

Cold Spring Harbor Laboratory

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