Defining the Parameters to Improve Plant Regeneration with Developmental Regulators

Author:

Nasti Ryan,Cody Jon P.,Zinselmeier Matt H.,Badey Nikil B.,Sridhar Adhvaith,Sharma Ambika,Maher Michael F.,Blackman Benjamin K.ORCID,Voytas Daniel F.

Abstract

AbstractTissue culture methods which serve as the standard to regenerate modified plants are challenging and have limited the capacity to engineer new accessions. To improve upon these techniques, genome modifying reagents have been combined with developmental regulators to create gene edited plant tissues in both monocot and eudicot species. Co-culturing seedlings with Agrobacterium strains encoding developmental regulatory genes has proved to be effective at producingde novomeristems in multiple eudicot species. In order to see that this technology scales well beyond proof of concept experiments, various parameters were tested for refinement. Improvements have been observed at the key stages of growth induction and progression to shooting by manipulating the vector design, developmental regulator choice, Agrobacterium strain selection, and regulatory gene removal systems. Having defined these parameters as viable optimization points, the avenues to apply developmental regulators for plant regeneration in more diverse species have become more feasible.

Publisher

Cold Spring Harbor Laboratory

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