Effect of Over-expression of GRXs on Thermo and Acetic Acid Stress Tolerance ofSaccharomyces cerevisiae

Abstract

AbstractEthanol production from renewable cellulosic materials is a globally significant research area. However, the high temperatures and acetic acid generated during cellulose pretreatment can inhibitSaccharomyces cerevisiaegrowth, reducing ethanol yields. This study investigates the impact of glutaredoxin family genes (GRXs) over-expression onS. cerevisiaecell growth and fermentation performance under thermal and acetic acid stress. Engineered strains overexpressingGRX1, GRX2, andGRX5demonstrated enhanced growth at 42°C, while those overexpressingGRX1, GRX2, GRX6, andGRX7showed improved growth at 1 g/L acetic acid. These results suggest that GRX over-expression can remediateS. cerevisiae, potentially accelerating advancements in green biomanufacturing.