Correlation of Remodeling Brain and Phenotype Behavior in the Comorbidity of the Chronic Obstructive Pulmonary Disease and Acute Cerebral Ischemia in Animal Model

Abstract

AbstractCognitive impairment is one of the most common features in patients with COPD, and is associated with COPD severity and comorbidities. Development of relevant models of respiratory-cerebrovascular comorbidity in human diseases is an important task of experimental medicine. The purpose of the research consisted in determination of the morphological criteria brain condition and their correlation with behavioral phenotype of animals in the experimental comorbidity of the chronic obstructive pulmonary disease (COPD) and acute cerebral ischemia (ACI). Modeling of COPD on the basis of the combination of inducers, reproducing a proteoclastic degradation of lung tissue and systemic inflammation, and modeling of ACI by the suture middle cerebral artery occlusion with to mimic ischemia condition were used. Comparative histological study of the brain, neurological and behavioral phenotype of animals was conducted. It has been shown that in case of COPD and ACI comorbidity, formation of neurogliovascular microstructural complexes in brain is more pronounced than at animals with isolated form of disease, which was indicative of active adaptive transformation of neocortex. Significant disturbance of neurological and behavioral status of animals under the conditions of COPD and ACI comorbidity was correlated with the structural changes in the microvascular layer and neurons of brain. This study provides new insights about formation of neurogliovascular complexes with altered quantitative ratio in the vessels that was indicative of the presence of pericellular and perivascular edemas of the brain, and correlating of the these changes with the behavior of animals.