Author:
Wang Zhaohui,Li Yang,Hou Bing,Pronobis Mira I.,Wang Yuemeng,Wang Mingqiao,Cheng Guangcun,Zhang Zhe,Weng Weining,Wang Yiqiang,Tang Yanfang,Xu Xuefan,Pan Rong,Lin Fei,Wang Nan,Chen Ziqing,Wang Shiwei,Ma Luyan zulie,Li Yangrui,Huang Dongliang,Jiang Li,Wang Zhiqiang,Zeng Wenfang,Zhang Ying,Du Xuemei,Lin Ying,Li Zhiqing,Xia Qingyou,Geng Jing,Dai Huaping,Wang Chen,Yu Yuan,Zhao Xiaodong,Yuan Zheng,Yan Jian,Ren Bing,Nie Qinghua,Zhang Xiquan,Wang Kun,Chen Fuling,Zhang Qin,Zhu Yuxian,Poss Kenneth D.,Tao Shengce,Meng Xun
Abstract
AbstractAntibodies are essential for elucidating the roles of genes decoded by genome sequencing. However, affordable technology for proteome-scale antibody generation does not exist. To address this, we developed the Proteome Epitope Tag Antibody Library (PETAL) and its array. PETAL consists of 62,208 mAbs against 15,199 peptides from diverse proteomes. PETAL harbors binders for a great multitude of proteins in nature due to antibody multispecificity, an intrinsic feature of an antibody. Distinctive combinations of 10,000-20,000 mAbs were found to target specific proteomes by array screening. Phenotype-specific mAb-target pairs were discovered for maize and zebrafish samples. Immunofluorescence and flow cytometry mAbs for human membrane proteins and ChIP-seq mAbs for transcription factors were identified from respective proteome-binding PETAL mAbs. Differential screening of cell surface proteomes of tumor and normal tissues discovered internalizing tumor antigens for antibody-drug conjugates. By discovering high affinity mAbs at a fraction of current time and cost, PETAL enables proteome-scale antibody generation and target discovery.
Publisher
Cold Spring Harbor Laboratory
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