Abstract
AbstractThe crucial role of microtubules in the mitotic-related segregation of chromosomes makes them an excellent target for anticancer microtubule targeting drugs (MTDs) such as vinflunine, colchicine, and docetaxel. MTDs affect mitosis by directly perturbing the structural organization of microtubules. By a direct assessment of the biomechanical properties of prostate cancer cells exposed to different MTDs using atomic force microscopy, we show that cell stiffening is a candidate mechanism through which cancer cells preserve the original phenotype in response to the application of MTDs. While changes in cellular rigidity are typically mainly attributed to remodeling of the actin filaments in the cytoskeleton, here we provide evidence that cell stiffening can be driven by a crosstalk between actin filaments and microtubules in drug-treated cells. Our findings improve the interpretation of biomechanical data obtained for living cells in studies of various physiological and pathological processes.
Publisher
Cold Spring Harbor Laboratory
Reference54 articles.
1. Actin, microtubules, and vimentin intermediate filaments cooperate for elongation of invadopodia
2. Microtubule assembly dynamics: An attractive target for anticancer drugs
3. Preface
4. Update on taxane development: New analogs and new formulations;Drug Design, Development and Therapy,2012
5. Vinca alkaloids;International Journal of Preventive Medicine,2013