Author:
Bae Sangwook,Jung Yushin,Kim Sungsik,Kim Jinhyun,Lee Amos Chungwon,Lee Dongsoon,Kwon Sunghoon
Abstract
AbstractAnalyzing archived peripheral blood smears is a potential route towards gaining cell morphology and genome information of blood cell types from various diseases. Yet, acquiring whole genome information from morphologically targeted cells was difficult, especially for rare cell types. The main causes for such difficulty were the inevitable usage of cell stains leading to whole genome amplification inhibition, and insufficient cell isolation performance of previously introduced laser microdissection (LMD) techniques. Here, we introduce a new laser-based cell isolation technique and a whole genome amplification (WGA) protocol optimized for whole genome analysis from minute input of hematologically stained cells. We were able to perform whole genome copy number profiling and SNP analysis from as little as 5 cells.
Publisher
Cold Spring Harbor Laboratory
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