Somatic Hybridization in the Moss Physcomitrella patens Using PEG-Induced Protoplast Fusion-Reference-Cited by-同舟云学术

Somatic Hybridization in the Moss Physcomitrella patens Using PEG-Induced Protoplast Fusion

Published:2009-02 Issue:2 Volume:2009 Page:pdb.prot5141
ISSN:1940-3402
Container-title:Cold Spring Harbor Protocols
language:en
Short-container-title:Cold Spring Harb Protoc

Author:

Cove David J.,Perroud Pierre-François,Charron Audra J.,McDaniel Stuart F.,Khandelwal Abha,Quatrano Ralph S.

Abstract

INTRODUCTIONAs an alternative to sexual crossing, protoplasts from two strains of moss (Physcomitrella patens) can be hybridized using polyethylene glycol (PEG). Although the efficiency is low, it requires no sophisticated apparatus. Hybrids are readily obtained using complementary auxotrophic mutants or strains with transgenic antibiotic resistance markers. It is now routine to obtain hybrids using transgenic strains that are hygromycin- or G418-resistant by selecting hybrids on medium containing both antibiotics.

Publisher

Cold Spring Harbor Laboratory

Subject

General Biochemistry, Genetics and Molecular Biology

Reference4 articles.

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2. Culturing the Moss Physcomitrella patens: Figure 1.

3. Isolation and Regeneration of Protoplasts of the Moss Physcomitrella patens: Figure 1.

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