Transformation of the Moss Physcomitrella patens Using Direct DNA Uptake by Protoplasts

Abstract

INTRODUCTIONThis protocol describes how to transform moss (Physcomitrella patens) protoplasts using polyethylene glycol (PEG)-mediated DNA uptake. The transformation rates for direct uptake by protoplasts of DNA with and without genomic sequence (a targeting construct) are typically 10−5 and 10−3, respectively. (These are the frequencies of stable transformants among regenerants surviving the transformation procedure.)