Author:
Cove David J.,Perroud Pierre-François,Charron Audra J.,McDaniel Stuart F.,Khandelwal Abha,Quatrano Ralph S.
Abstract
INTRODUCTIONThis article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by self-fertilization and sexual crossing.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
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