Author:
Cove David J.,Perroud Pierre-François,Charron Audra J.,McDaniel Stuart F.,Khandelwal Abha,Quatrano Ralph S.
Abstract
INTRODUCTIONThis article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by self-fertilization and sexual crossing.
Publisher
Cold Spring Harbor Laboratory
Subject
General Biochemistry, Genetics and Molecular Biology
Reference6 articles.
1. The isolation and preliminary characterisation of auxotrophic and analogue resistant mutants of the moss, Physcomitrella patens
2. Boyd P.J. Hall J. Cove D.J. (1988) in Methods in bryology. Proceedings of the Bryological Methods Workshop, Mainz, Germany, An airlift fermenter for the culture of the moss Physcomitrella patens , ed Glime J.M. (Hattori Botany Laboratory, Japan), pp 41–45.
3. Evidence for the restricted passage of metabolites into the sporophyte of the moss, Physcomitrella patens;Courtice;J. Bryol.,1978
4. The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies
5. Cryopreservation in the moss, Physcomitrella patens.;Grimsley;CryoLetters,1983
Cited by
63 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献