Author:
Gilley D,Lee M S,Blackburn E H
Abstract
The ribonucleoprotein enzyme telomerase synthesizes telomeric DNA by copying a template sequence in the telomerase RNA. We studied the functional roles of specific residues in the Tetrahymena telomerase RNA template region. Unexpectedly, mutation of certain templating residues caused dramatic effects on specific aspects of the enzyme reaction, including loss of enzymatic fidelity and premature product dissociation. None of these fundamental changes in enzymatic action are explainable by altered base-pairing between the telomerase RNA and DNA substrate. These influences of specific template bases of the telomerase RNA on enzymatic properties of telomerase provide evidence for critical roles of these RNA residues in two active site functions--fidelity and DNA substrate/enzyme interaction.
Publisher
Cold Spring Harbor Laboratory
Subject
Developmental Biology,Genetics
Reference37 articles.
1. Functional reconstitution of wild-type and mutant Tetrahymena telomerase.
2. Bebenek, K. and T.A. Kunkel. 1993. The fidelity of retroviral reverse transcriptases. In Reverse transcriptase. (ed. A.M. Skalka and S.P. Goff), pp. 85–103. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
3. Structure of DNA Polymerase I Klenow Fragment Bound to Duplex DNA
4. Chromosome ends in Drosophila without telomeric DNA sequences.
5. Architecture of telomerase RNA.;EMBO J.,1994
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