Marker-assisted pyramiding of two major broad-spectrum bacterial blight resistance genes,Xa21andXa33into an elite maintainer line of rice, DRR17B

Abstract

AbstractBacterial blight (BB) disease reduces the yield of rice varieties and hybrids considerably in many tropical rice growing countries like India. The present study highlights the development of durable BB resistance into the background of an elite maintainer of rice, DRR17B, by incorporating two major dominant genes,Xa21andXa33through marker-assisted backcross breeding (MABB). Through two sets of backcrosses, the two BB resistance genes were transferred separately to DRR17B. In this process, at each stage of backcrossing, foreground selection was carried out for the target resistance genes and for non-fertility restorer alleles concerning the major fertility restorer genesRf3andRf4, using gene-specific PCR-based markers, while background selection was done using a set of 61 and 64 parental polymorphic SSR markers respectively. Backcross derived lines possessing eitherXa21orXa33along with maximum genome recovery of DRR17B were identified at BC3F1generation and selfed to develop BC3F2s. Plants harboringXa21orXa33in homozygous condition were identified among BC3F2s and were intercrossed with each other to combine both the genes. The intercross F1plants (ICF1) were selfed and the intercross F2(ICF2) plants possessing bothXa21andXa33in homozygous condition were identified with the help of markers. They were then advanced further by selfing until ICF4generation. Selected ICF4lines were evaluated for their resistance against BB with eight virulent isolates and for key agro-morphological traits. Six promising two-gene pyramiding lines of DRR17B with high level of BB resistance and agro-morphological attributes similar or superior to DRR17B with complete maintenance ability have been identified. These lines with elevated level of durable resistance may be handy tool for BB resistance breeding.