HspA1BIs a Prognostic Biomarker and Correlated With Immune Infiltrates in different subtypes of Breast Cancers

Abstract

ABSTRACTBackgroundHeat shock A1B, also known as HSP70kDa protein 1B, encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family.HspA1Bis a critical gene which related to many type of diseases by involving in the ubiquitin-proteasome pathway. However, the correlations ofHspA1Bto prognosis and tumor-infiltrating lymphocytes in different cancers remain unclear.MethodsHspA1Bexpression was evaluated on the Oncomine database and Tumor Immune Estimation Resource (TIMER) site. We analyzed the influence ofHspA1Bon clinical prognosis using Kaplan-Meier plotter, the PrognoScan database and Gene Expression Profiling Interactive Analysis (GEPIA). The correlations betweenHspA1Band cancer immune infiltrates was investigated via TIMER. In addition, correlations betweenHspA1Bexpression and gene marker sets of immune infiltrates were analyzed by TIMER and GEPIA.ResultsThree cohorts (GSE9195, GSE9893, GSE3494-GPL96)) of breast cancer patients showed that highHspA1Bexpression was associated with poorer overall survival (OS), disease-specific survival (DSS), and disease-free survival (DFS). In addition, highHspA1Bexpression was significantly correlated with poor OS and progression-free survival (PFS) in bladder cancer, brain cancer and skin cancer. Moreover,HspA1Bsignificantly impacts the prognosis of diverse cancers via The Cancer Genome Atlas (TCGA).HspA1Bexpression was positively correlated with infiltrating levels of CD4+ T and CD8+ T cells, macrophages, neutrophils, and dendritic cells (DCs) indifferent subtypes of Breast cancer.HspA1Bexpression showed strong correlations with diverse immune marker sets in BRCA-Luminal.ConclusionsOur findings suggest thatHspA1Bis correlated with prognosis and immune infiltrating levels of, including those of CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and DCs in multiple cancers, especially in colon and gastric cancer patients. In addition,HspA1Bexpression potentially contributes to regulation of tumor-associated macrophages (TAMs), DCs, T cell exhaustion and Tregs in colon and gastric cancer. These findings suggest thatHspA1Bcan be used as a prognostic biomarker for determining prognosis and immune infiltration in BRCA-Luminal subtype.