Evaluation of cerebrospinal fluid alpha-synuclein seed amplification assay in PSP and CBS

Author:

Vaughan DP,Fumi R,Theilmann Jensen M,Georgiades T,Wu L,Lux D,Obrocki R,Lamoureux J,Ansorge O,Allinson KSJ,Warner TT,Jaunmuktane Z,Misbahuddin A,Leigh PN,Ghosh BCP,Bhatia KP,Church A,Kobylecki C,Hu MTM,Rowe JB,Blauwendraat C,Morris HR,Jabbari E

Abstract

AbstractBackgroundSeed amplification assay (SAA) testing has become an important biomarker in the diagnosis of alpha-synuclein related neurodegenerative disorders.ObjectivesTo assess the rate of alpha-synuclein SAA positivity in progressive supranuclear palsy (PSP) and corticobasal syndrome (CBS), and analyse the clinical and pathological features of SAA positive and negative cases.Methods106 CSF samples from clinically diagnosed PSP (n=59), CBS (n=37) and indeterminate parkinsonism cases (n=10) were analysed using alpha-synuclein SAA.ResultsThree cases (1 PSP, 2 CBS) were Multiple System Atrophy (MSA)-type SAA positive. 5/59 (8.5%) PSP cases were Parkinson’s disease (PD)-type SAA positive, and these cases were older and had a shorter disease duration compared with SAA negative cases. In contrast, 9/35 (25.7%) CBS cases were PD-type SAA positive.ConclusionsOur results suggest that PD-type seeds can be detected in PSP and CBS using a CSF alpha-synuclein SAA, and in PSP this may impact on clinical course.

Publisher

Cold Spring Harbor Laboratory

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