Abstract
AbstractWe present a new set of computational tools that enable accurate and widely applicable 3D segmentation of nuclei in various 3D digital organs. We developed a novel approach for ground truth generation and iterative training of 3D nuclear segmentation models, which we applied to popular CellPose, PlantSeg, and StarDist algorithms. We provide two high-quality models trained on plant nuclei that enable 3D segmentation of nuclei in datasets obtained from fixed or live samples, acquired from different plant and animal tissues, and stained with various nuclear stains or fluorescent protein-based nuclear reporters. We also share a diverse high-quality training dataset of about 10,000 nuclei. Furthermore, we advanced the MorphoGraphX analysis and visualization software by, among other things, providing a method for linking 3D segmented nuclei to their surrounding cells in 3D digital organs. We found that the nuclear-to-cell volume ratio varies between different ovule tissues and during the development of a tissue. Finally, we extended the PlantSeg 3D segmentation pipeline with a proofreading script that uses 3D segmented nuclei as seeds to correct cell segmentation errors in difficult-to-segment tissues.Summary StatementWe present computational tools that allow versatile and accurate 3D nuclear segmentation in plant organs, enable the analysis of cell-nucleus geometric relationships, and improve the accuracy of 3D cell segmentation.
Publisher
Cold Spring Harbor Laboratory
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