A bioinformatics screen reveals Hox and chromatin remodeling factors at theDrosophilahistone locus

Author:

Hodkinson Lauren J.ORCID,Smith Connor,Comstra H. SkyeORCID,Albanese Eric H.,Ajani Bukola A.,Arsalan Kawsar,Daisson Alvero Perez,Forrest Katherine B.,Fox Elijah H.,Guerette Matthew R.,Khan Samia,Koenig Madeleine P.,Lam Shivani,Lewandowski Ava S.,Mahoney Lauren J.,Manai Nasserallah,Miglay JonCarlo,Miller Blake A.,Milloway Olivia,Ngo Vu D.,Oey Nicole F.,Punjani Tanya A.,SiMa HaoMin,Zeng Hollis,Schmidt Casey A.ORCID,Rieder Leila E.ORCID

Abstract

AbstractCells orchestrate histone biogenesis with strict temporal and quantitative control. To efficiently regulate histone biogenesis, the repetitiveDrosophila melanogasterreplication-dependent histone genes are arrayed and clustered at a single locus. Regulatory factors concentrate in a nuclear body known as the histone locus body (HLB), which forms around the locus. Historically, HLB factors are largely discovered by chance, and few are known to interact directly with DNA. It is therefore unclear how the histone genes are specifically targeted for unique and coordinated regulation. To expand the list of known HLB factors, we performed a candidate-based screen by mapping 30 publicly available ChIP datasets and 27 factors to theDrosophilahistone gene array. We identified novel transcription factor candidates, including theDrosophilaHox proteins Ultrabithorax, Abdominal-A and Abdominal-B, suggesting a new pathway for these factors in influencing body plan morphogenesis. Additionally, we identified six other transcription factors that target the histone gene array: JIL-1, Hr78, the long isoform of fs(1)h as well as the generalized transcription factors TAF-1, TFIIB, and TFIIF. Our foundational screen provides several candidates for future studies into factors that may influence histone biogenesis. Further, our study emphasizes the powerful reservoir of publicly available datasets, which can be mined as a primary screening technique.

Publisher

Cold Spring Harbor Laboratory

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