The use of DNA barcoding for the identification of giant hogweeds in the European North-East of Russia

Abstract

AbstractQualitative differences in the nrDNA ITS and ETS sequences in the total sample were determined to allow identification of the following groups of samples:H. mantegazzianum, H. cf.sosnowskyi, H. cf.mantegazzianumandH. cf.sosnowskyi×H. cf.mantegazzianumamong other representatives of theHeracleumgenus presented in the genetic databases. The study revealed the absence of correlation between a sample type in the groupH. mantegazzianum, H. cf.sosnowskyi, H. cf.mantegazzianumandH. cf.sosnowskyi×H. cf.mantegazzianumand qualitative transformations in the ITS and ETS sequences. The qualitative analysis of the rbcL, matK, rps16 intron, intergenic spacers psbA-trnH, trnQ-rps16, rps16-trnK and rpl32-trnL cpDNA sequences for the samples identified asHeracleum mantegazzianum,H. cf.sosnowskyi,H. cf.mantegazzianumandH. cf.sosnowskyi×H. cf.mantegazzianumdemonstrated no correlation between them as well.