Tracking the intensity of the mechanism to produce antigenic diversity by subtelomeric ectopic recombination across the phylogeny ofPlasmodiumparasites

Abstract

1AbstractThe generation of antigenic diversity, key for parasite virulence, has been investigated in the genusPlasmodium, mainly inPlasmodium falciparum. Cytogenetic and molecular studies have revealed that its subtelomeres are rich in antigenic gene families and undergo ectopic recombination. As a result, these families are highly variable and even species-specific. More recent analyses focused on the phylogenetic mapping ofP. falciparumchromosomes with the bioinformatic tool PhyloChromoMap, showed that ectopic recombination of subtelomeres extends to all chromosomes. Although antigenic gene families have been described in subtelomeres of otherPlasmodiumspecies, the intensity of this mechanism in these species is still unclear. In this study, we investigated to what extent ectopic recombination of subtelomeres drives the generation of antigenic diversity in 19Plasmodiumspecies. To achieve this, we analyzed the profile of gene conservation in maps of all their chromosomes with PhyloChromoMap. Our results suggest that ectopic recombination of subtelomeres is more critical for the diversification ofpirorrif/stevorgenes than other antigenic gene families. Furthermore, its intensity varies among subgenera and was likely acquired and lost multiple times in the phylogeny ofPlasmodium.