Transcription activator WCC recruits deacetylase HDA3 to control transcription dynamics and bursting in Neurospora

Abstract

ABSTRACTRNA polymerase II initiates transcription either randomly or in bursts. We examined the light-dependent transcriptional activator White Collar Complex (WCC) ofNeurosporato characterize the transcriptional dynamics of the strongvivid(vvd) promoter and the weakerfrequency(frq) promoter. We show that WCC is not only an activator but also represses transcription by recruiting histone deacetylase 3 (HDA3). Our data suggest that bursts offrqtranscription are governed by a long-lived refractory state established and maintained by WCC and HDA3 at the core promoter, whereas transcription ofvvdis determined by WCC binding dynamics at an upstream activating sequence. Thus, in addition to stochastic binding of transcription factors, transcription factor-mediated repression may also influence transcriptional bursting.TEASERBalanced interaction of transcription factor with coactivator and corepressors determines transcription dynamics and bursting.