Molecular Diagnosis Of Clostridium piliforme, The Causative Agent Of Tyzzer’s Disease In Mice And Rats

Abstract

Tyzzer’s disease, caused by Clostridium piliforme (C. piliforme), occurs in many species of mammals mainly in rodents, rabbits and foals is an acute, epizootic bacterial disease that can result in mortality. In our study, C. piliforme was investigated in feces samples from mice and rats in a laboratory experimental animals center by PCR technique. C. piliforme was detected in 44 (83.01%) of a total of 53 mouse feces of different ages. Of the mice found positive, 28 (73.6%) were female and 16 (88.8%) were male. C. piliforme was detected in 40 (90.9%) of a total of 44 rats feces of different ages. Of the rats found positive, 25 (96.1%) were female and 15 (83.3%) were male. C. piliforme, the causative agent of Tyzzer's disease, was detected at a high rate in mice and rats examined by the classical PCR method. When evaluated in terms of gender, 28 (73.6%) of the 40 mice positive for Tyzerr's disease were female and 16 (88.8%) were male; While 25 (96.1%) of the rats were female, 15 (83.3%) were male. As a result, Tyzerr's disease caused by C. piliforme was detected at a high rate in mice and rats by classical PCR. When evaluated by age, the disease was detected to be higher in mice at the ages of 4, 5 and 8, and in rats at the ages of 4, 5, 6 and 7. Due to the reliability of the analyses and the high risk of transmission to other animals in centers where laboratory animals are raised, Tyzzer's disease should be checked and necessary precautions should be taken to prevent the disease.