Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media-Reference-Cited by-同舟云学术

Identifying the effect of vancomycin on health care–associated methicillin-resistant Staphylococcus aureus strains using bacteriological and physiological media

Published:2021-01 Issue:1 Volume:10 Page:
ISSN:2047-217X
Container-title:GigaScience
language:en
Short-container-title:

Author:

Rajput Akanksha¹,Poudel Saugat¹,Tsunemoto Hannah²,Meehan Michael³⁴,Szubin Richard¹,Olson Connor A¹,Seif Yara¹,Lamsa Anne²,Dillon Nicholas⁵⁶,Vrbanac Alison⁵⁶,Sugie Joseph²,Dahesh Samira⁵⁶,Monk Jonathan M¹,Dorrestein Pieter C³⁴⁷⁸,Knight Rob¹⁵⁸⁹^ORCID,Pogliano Joe²,Nizet Victor⁴⁵⁶⁸,Feist Adam M¹¹⁰,Palsson Bernhard O¹⁵⁸¹⁰^ORCID

Affiliation:

1. Department of Bioengineering, University of California, 9500 Gilman Dr, La Jolla, CA 92093, USA

2. Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA

3. Collaborative Mass Spectrometry Innovation Center, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA

4. Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA

5. Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92023, USA

6. Collaborative to Halt Antibiotic-Resistant Microbes (CHARM), Department of Pediatrics, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA

7. Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA

8. Center for Microbiome Innovation, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA

9. Department of Computer Science and Engineering, University of California San Diego, 9500 Gilman Dr, La Jolla, CA 92093, USA

10. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, Building 220, 2800 Kongens, Lyngby, Denmark

Abstract

Abstract Background The evolving antibiotic-resistant behavior of health care–associated methicillin-resistant Staphylococcus aureus (HA-MRSA) USA100 strains are of major concern. They are resistant to a broad class of antibiotics such as macrolides, aminoglycosides, fluoroquinolones, and many more. Findings The selection of appropriate antibiotic susceptibility examination media is very important. Thus, we use bacteriological (cation-adjusted Mueller-Hinton broth) as well as physiological (R10LB) media to determine the effect of vancomycin on USA100 strains. The study includes the profiling behavior of HA-MRSA USA100 D592 and D712 strains in the presence of vancomycin through various high-throughput assays. The US100 D592 and D712 strains were characterized at sub-inhibitory concentrations through growth curves, RNA sequencing, bacterial cytological profiling, and exo-metabolomics high throughput experiments. Conclusions The study reveals the vancomycin resistance behavior of HA-MRSA USA100 strains in dual media conditions using wide-ranging experiments.

Funder

National Institutes of Health

Publisher

Oxford University Press (OUP)

Subject

Computer Science Applications,Health Informatics

Link

http://academic.oup.com/gigascience/article-pdf/10/1/giaa156/35558770/giaa156.pdf

Reference42 articles.

1. Incidence, prevalence, and management of MRSA bacteremia across patient populations-a review of recent developments in MRSA management and treatment;Hassoun;Crit Care,2017

2. Epidemiology of nosocomial infections caused by methicillin-resistant Staphylococcus aureus;Thompson;Ann Intern Med,1982

3. Waves of resistance: Staphylococcus aureus in the antibiotic era;Chambers;Nat Rev Microbiol,2009