Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification
Author:
Publisher
Oxford University Press (OUP)
Subject
Genetics
Link
http://academic.oup.com/nar/article-pdf/36/15/e92/18780227/gkn420.pdf
Reference19 articles.
1. Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by ‘reconditioning PCR’;Thompson;Nucleic Acids Res,2002
2. Amplified RNA synthesized from limited quantities of heterogeneous cDNA;Van-Gelder;Proc. Natl Acad. Sci. USA,1990
3. Antisense RNA Amplification: A Linear Amplification Method for Analyzing the mRNA Population from Single Living Cells;Phillips;Methods,1996
4. Linear mRNA amplification from as little as 5 ng total RNA for global gene expression analysis;Dafforn;Biotechniques,2004
5. Optimized RNA amplification using T7-RNA-polymerase based in vitro transcription;Moll;Anal. Biochem,2004
Cited by 9 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
1. Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis;BMC Research Notes;2015-06-20
2. Single prokaryotic cell isolation and total transcript amplification protocol for transcriptomic analysis;Nature Protocols;2015-06-04
3. Single-cell analysis of the transcriptome and its application in the characterization of stem cells and early embryos;Cellular and Molecular Life Sciences;2014-03-21
4. Fluorescence laser microdissection reveals a distinct pattern of gene activation in the mouse hippocampal region;Scientific Reports;2012-11-07
5. A simple and novel method for RNA-seq library preparation of single cell cDNA analysis by hyperactive Tn5 transposase;Developmental Dynamics;2012-08-28
1.学者识别学者识别
2.学术分析学术分析
3.人才评估人才评估
"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370
www.globalauthorid.com
TOP
Copyright © 2019-2024 北京同舟云网络信息技术有限公司 京公网安备11010802033243号 京ICP备18003416号-3