Affiliation:
1. 4736 N. Tripp Ave., Chicago, Ill. 60630
Abstract
Abstract
A simple dye-binding method for determining serum albumin, which employs the dye, 2-(4'-hydroxybenzeneazo) benzoic acid (HBABA), is described. Albumin in 0.1 ml. of serum is determined colorimetrically at 510 mµ. Reliability of the method has been established by comparison with another method using HBABA and with a salt fractionation-biuret method, by recovery experiments, variation in ambient temperature, demonstration of optimal dye molarity and pH, and replicate analyses.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
12 articles.
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