Separation of serum creatine kinase isoenzymes by ion-exchange column chromatography.-Reference-Cited by-同舟云学术

Separation of serum creatine kinase isoenzymes by ion-exchange column chromatography.

Published:1977-03-01 Issue:3 Volume:23 Page:504-510
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Klein B,Foreman J A,Jeunelot C L,Sheehan J E

Abstract

Abstract We describe a practical, technically convenient DEAE-Sephadex chromatographic column and a three-fraction salt-gradient elution procedure, with which serum creatine kinase activity is rapidly and quantitatively separated into the isoenzyme components with excellent analytical recovery of the total activity applied. The homogeneity of the isoenzyme fractions was demonstrated by electrophoresis and rechromatography. Sera assessed by the Mercer fractionation [Clin. Chem. 20, 36 (1974)] and the present procedure showed excellent agreement.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry (medical),Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/23/3/504/32841270/clinchem0504.pdf

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