Albumin Quantitation by Dye Binding and Salt Fractionation Techniques

Abstract

Abstract Two dye-binding reagents, 2-(4'-hydroxybenzeneazo)benzoic acid (HABA) and bromcresol green (BCG), for the quantitation of serum albumin have been compared with the biuret colorimetric procedure after salt fractionation. The linear regression equations for the biuret-BCG and the biuret-HABA comparisons were y = 0.43 + 0.91x and y = -0.45 + 1.15x, respectively. Their corresponding correlation coefficients were 0.93 and 0.83, respectively. The effects of icterus, lipemia, and hemolysis on the dye-binding procedures were generally greater in the case of the HABA procedure. A comparison of results with BCG and HABA for 20 sera having A/G ratios of less than 1, indicates them to be equally reliable.