Affiliation:
1. Department of Biochemistry of the Michael Reese Hospital and Medical Center, 29th St. and Ellis Ave., Chicago, Ill. 60616
Abstract
Abstract
A procedure is described for the assay of cystine in urine. Cystine is ad-sorbed on a column of Dowex-50 resin at pH 2, which is then washed with water and eluted with sodium acetate solution. Color is developed with 1,2-naphthoquinone-4-sulfonate. Homocystine does not interfere in the reaction. Routine reproducibility in the laboratory was ±10.3% (2 SD) for 24 normal urines, which contained 8 to 86 mg cystine per 24 h (those values falling within 2 SD). Mean recovery was 94.5%. In two cases in which the results were compared with those obtained with the amino acid analyzer, the method was indicated to be valid for screening urine to detect cystinuria
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
3 articles.
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