Affiliation:
1. Departments of Pathology and Clinical Laboratories, The University of Michigan, 1335 E. Catherine St., Ann Arbor, Mich. 48104
Abstract
Abstract
The adaptation of a tetrazolium salt assay for serum lactate dehydrogenase to fullautomation is described. The tetrazolium salt, INT, (2-p-iodophenyl-3-p-nitrophenyl-5-phenyl tetrazolium chloride) serves as the chromogenic indicator of the enzyme activity. Phenazine methosulfate serves as an intermediate electron carrier.
Estimation requires only 5 min. of incubation, and assays may be run at the rate of 60/hr. Comparison against the manual version of the assay with respect to normal and abnormal serum activity levels is excellent.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry (medical),Clinical Biochemistry
Cited by
14 articles.
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