Affiliation:
1. Bio-Science Laboratories, 2231 S. Carmelina Ave., Los Angeles, Calif
Abstract
Abstract
The specificity of the lipolytic activity occurring in sera from patients with acute pancreatitis for olive oil has been demonstrated. The "lipase" method proposed by Goldstein and co-workers, employing tributyrin as substrate, does not measure "pancreatitis lipase" "Pancreatitis lipase" differs from the enzymes present in pancreatic extracts so that, if the former is the subject of concern in experimental studies, sera from patients with pancreatitis must be used as the source of the enzyme.
Several variables involved in the use of olive oil as substrate have been examined, and the technic finally arrived at is essentially that proposed by Cherry and Crandall with the exception of the use of thymolphthalein as indicator in the titration. Normal values for this technic were determined.
Publisher
Oxford University Press (OUP)
Subject
Biochemistry, medical,Clinical Biochemistry
Cited by
27 articles.
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