Gas Chromatographic/Thin-Layer Chromatographic Analysis of Acetylated Codeine and Morphine in Urine-Reference-Cited by-同舟云学术

Gas Chromatographic/Thin-Layer Chromatographic Analysis of Acetylated Codeine and Morphine in Urine

Published:1975-09-01 Issue:10 Volume:21 Page:1486-1489
ISSN:0009-9147
Container-title:Clinical Chemistry
language:en
Short-container-title:

Author:

Jain Naresh C¹,Sneath Thomas C²,Budd Robert D²,Leung Wai J²

Affiliation:

1. The Departments of Pharmacology and of Community Medicine and Public Health, University of Southern California School of Medicine, Los Angeles, Calif. 90033.Rancho Los Amigos Hospital, 7601 E. Imperial Highway, Downey, Calif. 90242

2. Rancho Los Amigos Hospital, 7601 E. Imperial Highway, Downey, Calif. 90242

Abstract

Abstract This procedure positively identifies codeine and morphine in urine. Urine samples are hydrolyzed and extracted with organic solvent, and the extracts are evaporated and acetylated. The presence of codeine and morphine is ascertained by gas chromatography (3% OV-25 and 3% Poly-A 103 columns) and confirmed by thin-layer chromatography (system: ethyl acetate/acetone/concd ammonium hydroxide, 100/10/4.5 by vol; reagent: iodoplatinate). As little as 0.5 mg each of codeine and morphine per liter, in free and conjugated forms, is detectable by this method.

Publisher

Oxford University Press (OUP)

Subject

Biochemistry, medical,Clinical Biochemistry

Link

http://academic.oup.com/clinchem/article-pdf/21/10/1486/32809615/clinchem1486.pdf

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4. Gas chromatographic-mass spectrometric procedures used for the identification and determination of morphine, codeine and 6-monoacetylmorphine;Journal of Chromatography A;1994-07

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