Affiliation:
1. Miyazaki University, Faculty of Agriculture, Department of Veterinary Public Health, Kibanadai-Nishi, Gakuen, Miyazaki-shi 889-21, Japan
Abstract
Abstract
A rapid and sensitive liquid chromatographic (LC) method with fluorometric detection was developed to detect sulfadiazine, sulfathiazole, sulfamethazine, sulfamonomethoxine, sulfamethoxazole, and sulfadimethoxine residues in bovine serum and milk. p-Aminobenzoic acid (PABA) was added as an internal standard. The sulfonamides were extracted from samples and derivatized with fluorescamine, and 50 μL was injected into a NovaPak C18 LC column and eluted with acetonitrile– 10 mM potassium phosphate (30 + 70, v/v). The sulfonamides were detected fluorometrically (excitation, 390 nm; emission, 475 nm), and their retention times ranged from 6.2 to 16.5 min without interference from coextractives. The detection limit for standard sulfonamide solution was 0.1 ng/mL; the calibration curves were linear between 1 and 100 ng/mL in the presence of PABA as internal standard. Recovery rates of sulfonamides from spiked samples (1 and 10 ppb) were 95.4–107.2 and 81.4–89.6% for serum and 80.7–91.1 and 62.6–84.1% for milk, respectively.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
14 articles.
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