Affiliation:
1. Indian Statistical Institute, 203 B.T. Rd, Calcutta 700035, India
2. Indian Institute of Chemical Biology, 4 Raja S.C. MuUick Rd, Calcutta 700032, India
Abstract
Abstract
We have developed an improved method, using 96-well microtiter plates, for the microbiological assay of folic acid. With this method, the tedium of conventional microbiological analysis is substantially decreased. Culture volumes have been reduced 33-fold, and pipetting procedures have been simplified. Assay time has been reduced to 14 h, and sensitivity has increased 10-fold (0.1 ng/mL). Analytical recoveries range from 98 to 104%. Intra-assay and interassay variabilities are less than 11%. The assay does not require extensive manipulation of inoculum. Day-to-day variability has been minimized by using saline aliquots of the bacterial suspension stored at 4°C. The procedure is accurate, selective, and useful for direct measurement of folic acid in multivitamin formulations.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
9 articles.
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