Design and evaluation of an incoherent feed-forward loop for an arsenic biosensor based on standard iGEM parts

Author:

Barone Federico1,Dorr Francisco1,Marasco Luciano E1,Mildiner Sebastián1,Patop Inés L1,Sosa Santiago1,Vattino Lucas G1,Vignale Federico A1,Altszyler Edgar1,Basanta Benjamin1,Carlotto Nicolás1,Gasulla Javier1ORCID,Giménez Manuel1,Grande Alicia1,Nieto Moreno Nicolás1,Bonomi Hernán R1,Nadra Alejandro D1ORCID

Affiliation:

1. Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, iGEM 2013 Buenos Aires Team, Buenos Aires, Argentina

Abstract

Abstract The diversity and flexibility of life offers a wide variety of molecules and systems useful for biosensing. A biosensor device should be robust, specific and reliable. Inorganic arsenic is a highly toxic water contaminant with worldwide distribution that poses a threat to public health. With the goal of developing an arsenic biosensor, we designed an incoherent feed-forward loop (I-FFL) genetic circuit to correlate its output pulse with the input signal in a relatively time-independent manner. The system was conceived exclusively based on the available BioBricks in the iGEM Registry of Standard Biological Parts. The expected behavior in silico was achieved; upon arsenic addition, the system generates a short-delayed reporter protein pulse that is dose dependent to the contaminant levels. This work is an example of the power and variety of the iGEM Registry of Standard Biological Parts, which can be reused in different sophisticated system designs like I-FFLs. Besides the scientific results, one of the main impacts of this synthetic biology project is the influence it had on team’s members training and career choices which are summarized at the end of this article.

Funder

Ministerio de Ciencia, Tecnología e Innovación Productiva

Publisher

Oxford University Press (OUP)

Subject

Agricultural and Biological Sciences (miscellaneous),Biomedical Engineering,Biomaterials,Bioengineering,Biotechnology

Reference17 articles.

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