The minimum information required for a glycomics experiment (MIRAGE): reporting guidelines for capillary electrophoresis

Author:

Lageveen-Kammeijer Guinevere S M1,Rapp Erdmann23,Chang Deborah4,Rudd Pauline M5,Kettner Carsten6,Zaia Joseph4ORCID

Affiliation:

1. Center for Proteomics and Metabolomics, Leiden University Medical Center , Albinusdreef 2, 2300 RA Leiden, Netherlands

2. Max Planck Institute for Dynamics of Complex Technical Systems , Sandtorstraße 1, 39106 Magdeburg, Germany

3. glyXera GmbH , Brenneckestrasse 20—ZENIT, 39120 Magdeburg, Germany

4. Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University Medical Campus , 715 Albany Street, Boston, MA 02118, United States

5. Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR) , 20 Biopolis Way, 138668 Centros, Singapore

6. Beilstein-Institut , Trakehner Str. 7-9, 60487 Frankfurt am Main, Germany

Abstract

Abstract The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative to standardize the reporting of glycoanalytical methods and to assess their reproducibility. To date, the MIRAGE Commission has published several reporting guidelines that describe what information should be provided for sample preparation methods, mass spectrometry methods, liquid chromatography analysis, exoglycosidase digestions, glycan microarray methods, and nuclear magnetic resonance methods. Here, we present the first version of reporting guidelines for glyco(proteo)mics analysis by capillary electrophoresis (CE) for standardized and high-quality reporting of experimental conditions in the scientific literature. The guidelines cover all aspects of a glyco(proteo)mics CE experiment including sample preparation, CE operation mode (CZE, CGE, CEC, MEKC, cIEF, cITP), instrument configuration, capillary separation conditions, detection, data analysis, and experimental descriptors. These guidelines are linked to other MIRAGE guidelines and are freely available through the project website https://www.beilstein-institut.de/en/projects/mirage/guidelines/#ce_analysis (doi:10.3762/mirage.7).

Funder

MIRAGE

Publisher

Oxford University Press (OUP)

Subject

Biochemistry

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