Affiliation:
1. Department of Radiobiology , Faculty of Military Health Sciences, University of Defence, Hradec Králové, Czech Republic
Abstract
AbstractThis study establishes a new experimental approach for retrospective biodosimetric assessment by apoptosis detection ex vivo. For this purpose, we used mononuclear blood leukocytes isolated from the peripheral blood of irradiated Wistar rats and cultured them ex vivo for posterior analysis. Using flow cytometry, we distinguished apoptotic lymphocyte subsets individual biodosimetric potential at different time periods after exposure: B-lymphocytes 6–8 h (0–7 Gy), natural killer cells 24 h (0–7 Gy) and T-lymphocytes 24 h (0–1 Gy). This novel experimental design innovates through the need of a single blood sample from irradiated individuals for a complete biodosimetric assessment.
Publisher
Oxford University Press (OUP)
Subject
Public Health, Environmental and Occupational Health,Radiology, Nuclear Medicine and imaging,General Medicine,Radiation,Radiological and Ultrasound Technology
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