Antimicrobial and anthelmintic activities of the ethanolic extract, fractions and isolated compounds from Manilkara zapota L. P. Royen (Sapotaceae)

Author:

Mourão Mulvaney Layany Carolyny12,Xavier-Júnior Francisco Humberto3ORCID,Rodrigues Alice M S4,Stien Didier24,Allegretti Silmara Marques5,Garcia Vera Lúcia1

Affiliation:

1. Chemical, Biological and Agricultural Pluridisciplinary Research Center, University of Campinas, Campinas, SP, Brazil

2. CNRS- Institut de Chimie des Substances Naturelles, Gif-sur-Yvette, France

3. Programa de Pós-Graduação em Biotecnologia, Universidade Potiguar (UnP), Campus Salgado Filho, Natal, RN, Brazil

4. Sorbonne Universités, UPMC Univ Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM), Observatoire Océanologique, Banyuls-sur-mer, France

5. Department of Animal Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil

Abstract

Abstract Objectives To evaluate the antibacterial, antifungal and anthelmintic activities of the ethanolic extract (EEMz), fractions (LPFMz and HPFMz) and compounds isolated from the leaves of Manilkara zapota L. P. Royen. Methods Extract and fractions were produced by turbolization. LPFMz fraction was analysed by gas chromatography–mass spectrometry. The isolated compounds from HPFMz were purified by flash and preparative chromatographic methods, and chemically characterised by UPLC-ESITOFMS, optical rotation, and one- and two-dimensional 1H and 13C NMR techniques. Anthelmintic against Strongyloides venezuelensis and antimicrobial activities against Candida albicans, Trichophyton rubrum and Staphylococcus aureus were evaluated. Key findings EEMz showed mainly phenolic compounds and pentacyclic triterpenes from Δ12-oleane/Δ12-ursane series. Chlorogenic acid, myricetin-3-O-β-D-glucopyranoside, mearnsitrin, germanicol and germanicol acetate were reported to M. zapota leaves for the first time in this work. EEMz, HPFMz, LPFMz showed significative activity against C. albicans (16 µg/mL), while isolated flavonoids were active against S. aureus (<32 µg/mL). EEMz, phenolic-rich compounds (F2), and chlorogenic acid were potentially active against S. venezuelensis at 24 h. Conclusions M. zapota and its bioactive compound can be eligible such as a potential phytomedicine for the treatment of microbial and strongyloidiasis drug-resistant infections.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

São Paulo Research Foundation

Publisher

Oxford University Press (OUP)

Subject

Pharmaceutical Science,Pharmacology

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