Diversity of <i>Staphylococcus aureus</i> isolated from nares of ruminants-Reference-Cited by-同舟云学术

Diversity of Staphylococcus aureus isolated from nares of ruminants

Published:2023-12-30 Issue:1 Volume:135 Page:
ISSN:1365-2672
Container-title:Journal of Applied Microbiology
language:en
Short-container-title:

Author:

Loncaric Igor¹,Keinprecht Helga¹²,Irimaso Emmanuel³^ORCID,Cabal-Rosel Adriana⁴,Stessl Beatrix⁵,Ntakirutimana Christophe²,Marek Lydia¹²,Fischer Otto W²,Szostak Michael P¹,Oberrauch Celine¹,Wittek Thomas⁶,Müller Elke⁷⁸,Desvars-Larrive Amelie⁹¹⁰,Feßler Andrea T¹¹¹²,Braun Sascha D⁷⁸,Schwarz Stefan¹¹¹²,Ehling-Schulz Monika¹,Monecke Stefan⁷⁸¹³,Ehricht Ralf⁷⁸¹⁴,Ruppitsch Werner⁴,Grunert Tom¹^ORCID,Spergser Joachim¹

Affiliation:

1. Institute of Microbiology, University of Veterinary Medicine Vienna , Vienna 1210 , Austria

2. New Vision Veterinary Hospital (NVVH) Northern Province , Musanze district, Rwaza sector , Rwanda

3. School of Veterinary Medicine—CAVM, University of Rwanda , Nyagatare Campus, P.O. Box 210, Nyagatare , Rwanda

4. Austrian Agency for Health and Food Safety (AGES), Institute of Medical Microbiology and Hygiene , Vienna 1090 , Austria

5. Unit of Food Microbiology, Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna , Vienna 1210 , Austria

6. Clinical Unit of Ruminant Medicine, University of Veterinary Medicine , Vienna 1210 , Austria

7. Leibniz Institute of Photonic Technology (IPHT) , Jena 07745 , Germany

8. InfectoGnostics Research Campus , Jena 07743 , Germany

9. Unit of Veterinary Public Health and Epidemiology, Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine Vienna , Vienna 1210 , Austria

10. Complexity Science Hub Vienna , Vienna 1080 , Austria

11. Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin , Berlin 14163 , Germany

12. Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universität Berlin , Berlin 14163 , Germany

13. Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinik Dresden , Dresden 01307 , Germany

14. Institute of Physical Chemistry, Friedrich Schiller University Jena , Jena 07743 , Germany

Abstract

Abstract Aims To examine the diversity of Staphylococcus aureus isolated from nasal swabs of ruminants in Rwanda. Methods and results A total of 454 nasal swabs from 203 cows, 170 goats, and 81 sheep were examined for the presence of S. aureus, and 30 S. aureus isolates were detected and characterized pheno- and genotypically. Resistance to penicillin and/or tetracycline was observed. The isolates were assigned to eight different spa types (t21057 (novel), t10103, t18853, t20842, t318, t355, t458, and t9432) belonging to six clonal complexes (CCs) (CC152, CC30, CC3591, CC3666, CC522, and CC97). Panton–Valentine leukocidin (PVL) genes (lukF-PV/lukS-PV), the bovine leukocidin genes (lukM/lukF-P83), and the human and bovine variants of the toxic shock syndrome toxin gene tst-1 variants were detected. Conclusion These findings demonstrate that the nares of ruminants in Rwanda are colonized with mastitis-associated S. aureus, including lineages that are also carried by humans, underscoring the zoonotic risk, especially for livestock keepers. These results highlight the crucial importance of hygiene measures when handling livestock.

Funder

Veterinaer Projekt Ruanda

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,General Medicine,Biotechnology

Link

https://academic.oup.com/jambio/advance-article-pdf/doi/10.1093/jambio/lxad304/54930750/lxad304.pdf

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