Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers

Author:

Briggs Adrian W.1,Rios Xavier1,Chari Raj1,Yang Luhan1,Zhang Feng1,Mali Prashant1,Church George M.1

Affiliation:

1. Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115 and 2Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA 02142, USA

Publisher

Oxford University Press (OUP)

Subject

Genetics

Reference29 articles.

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2. Generating a synthetic genome by whole genome assembly: phiX174 bacteriophage from synthetic oligonucleotides;Smith;Proc. Natl. Acad. Sci. USA,2003

3. Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides;Stemmer;Gene,1995

4. Gene synthesis machines: DNA chemistry and its uses;Caruthers;Science,1985

5. Synthesis of high-quality libraries of long (150mer) oligonucleotides by a novel depurination controlled process;LeProust;Nucleic Acids Res.,2010

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