HSP70 alleviates sepsis-induced cardiomyopathy by attenuating mitochondrial dysfunction-initiated NLRP3 inflammasome-mediated pyroptosis in cardiomyocytes

Author:

Song Chenlu1ORCID,Zhang Yiqiu1,Pei Qing1ORCID,Zheng Li2,Wang Meiyu2,Shi Youzhen3,Wu Shan1,Ni Wei4,Fu Xiujun1,Peng Yinbo1,Zhang Wen1,Yao Min156

Affiliation:

1. Shanghai Jiao Tong University School of Medicine Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, , 639 Zhizaoju Road, Shanghai, 200011 , China

2. Baoshan Integrated Traditional Chinese and Western Medicine Hospital Department of Ultrasound, , 28 Tuanjie Road, Shanghai, 201999 , China

3. Shanghai Jiao Tong University School of Medicine Department of Ultrasound, Shanghai Ninth People's Hospital, , 280 Mohe Road, Shanghai, 201999 , China

4. Huazhong University of Science and Technology Wuhan National Laboratory for Optoelectronics, , 1037 Luoyu road, Wuhan, 430000 , China

5. Institute of Traumatic Medicine , , 201999 , China

6. Shanghai Jiao Tong University School of Medicine Shanghai , , 201999 , China

Abstract

Abstract Background Sepsis-induced cardiomyopathy (SIC) is an identified serious complication of sepsis that is associated with adverse outcomes and high mortality. Heat shock proteins (HSPs) have been implicated in suppressing septic inflammation. The aim of this study was to investigate whether HSP70 can attenuate cellular mitochondrial dysfunction, exuberated inflammation and inflammasome-mediated pyroptosis for SIC intervention. Methods Mice with cecal ligation plus perforation (CLP) and lipopolysaccharide (LPS)-treated H9C2 cardiomyocytes were used as models of SIC. The mouse survival rate, gross profile, cardiac function, pathological changes and mitochondrial function were observed by photography, echocardiography, hematoxylin–eosin staining and transmission electron microscopy. In addition, cell proliferation and the levels of cardiac troponin I (cTnI), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were determined by Cell Counting Kit-8, crystal violet staining and enzyme-linked immunosorbent assay. Moreover, mitochondrial membrane potential was assessed by immunofluorescence staining, and dynamin-related protein 1 and pyroptosis-related molecules [nucleotide-binding domain, leucine-rich-repeat containing family pyrin domain-containing 3 (NLRP3), caspase-1, gasdermin-D (GSDMD), gasdermin-D N-terminal (GSDMD-N)] were measured by western blotting, immunoprecipitation and immunoblotting. Finally, hsp70.1 knockout mice with CLP were used to verify the effects of HSP70 on SIC and the underlying mechanism. Results Models of SIC were successfully established, as reduced consciousness and activity with liparotrichia in CLP mice were observed, and the survival rate and cardiac ejection fraction (EF) were decreased; conversely, the levels of cTnI, TNF-α and IL-1β and myocardial tissue damage were increased in CLP mice. In addition, LPS stimulation resulted in a reduction in cell viability, mitochondrial destabilization and activation of NLRP3-mediated pyroptosis molecules in vitro. HSP70 treatment improved myocardial tissue damage, survival rate and cardiac dysfunction caused by CLP. Additionally, HSP70 intervention reversed LPS-induced mitochondrial destabilization, inhibited activation of the NLRP3 inflammasome, caspase-1, GSDMD and GSDMD-N, and decreased pyroptosis. Finally, knockout of hsp70.1 mice with CLP aggravated cardiac dysfunction and upregulated NLRP3 inflammasome activity, and exogenous HSP70 significantly rescued these changes. It was further confirmed that HSP70 plays a protective role in SIC by attenuating mitochondrial dysfunction and inactivating pyroptotic molecules. Conclusions Our study demonstrated that mitochondrial destabilization and NLRP3 inflammasome activation-mediated pyroptosis are attributed to SIC. Interestingly, HSP70 ameliorates sepsis-induced myocardial dysfunction by improving mitochondrial dysfunction and inhibiting the activation of NLRP3 inflammasome-mediated pyroptosis, and such a result may provide approaches for novel therapies for SIC.

Funder

National Natural Science Foundation of China

Publisher

Oxford University Press (OUP)

Subject

Critical Care and Intensive Care Medicine,Dermatology,Biomedical Engineering,Emergency Medicine,Immunology and Allergy,Surgery

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