Nutrient intake and influence on markers of oxidative stress in zoo-managed male snow leopards (Uncia uncia)

Abstract

Abstract Oxidative stress (OS) results from the overproduction of reactive species. Nutrient intake can contribute positively or negatively to OS, and the lack of established nutrient requirements for most of the exotic species managed in zoos exacerbates the possibilities for nutrient imbalances that potentially could lead to reactive species production. The objective of this study was to evaluate the influence of nutrient intake and nutritional husbandry on markers of OS in male snow leopards (n = 14) maintained in U.S. facilities (n = 12). Diet samples and husbandry information were obtained and snow leopards were immobilized once for collection of blood. Samples were analyzed for chemical composition (diet and blood), antioxidant capacity (blood), and markers of OS (blood). Correlations between weekly nutrient intakes and markers of OS were analyzed by linear regression. Analyzed markers of OS included antioxidant enzymes (superoxide dismutase [SOD] and glutathione peroxidase [GPx]) and ferric reducing antioxidant potential that are protective against OS, and protein carbonyls, thiobarbituric acid reactive substances, and DNA/RNA damage that are indicative of oxidative damage. Weekly copper intake (10.1 to 80.2 mg) was negatively correlated with DNA/RNA damage (R2 = 0.44; P = 0.01). Weekly sodium intake (4.4 to 12.7 g) was positively correlated with GPx activity (R2 = 0.43; P = 0.04). More frequent feeding of whole prey (0.3 to 3 times/wk) was correlated with increased blood SOD activity (R2 = 0.55; P < 0.01). In conclusion, greater dietary copper intake and more frequent feeding of whole prey may reduce OS in snow leopards. Dietary sodium intake and relationship with GPx activity should be further evaluated to determine benefit or detriment. No cause and effect can be inferred from our results, but our data suggest altering dietary form and nutrient concentrations may influence OS in snow leopards.